eprintid: 7844 rev_number: 6 eprint_status: archive userid: 2 dir: disk0/00/00/78/44 datestamp: 2023-07-06 23:30:07 lastmod: 2023-07-06 23:30:07 status_changed: 2023-07-06 23:30:07 type: article metadata_visibility: show creators_name: Cianciosi, Danila creators_name: Armas Diaz, Yasmany creators_name: Alvarez-Suarez, José M. creators_name: Chen, Xiumin creators_name: Zhang, Di creators_name: Martínez López, Nohora Milena creators_name: Briones Urbano, Mercedes creators_name: Quiles, José L. creators_name: Amici, Adolfo creators_name: Battino, Maurizio creators_name: Giampieri, Francesca creators_id: creators_id: creators_id: creators_id: creators_id: creators_id: nohora.martinez@uneatlantico.es creators_id: mercedes.briones@uneatlantico.es creators_id: jose.quiles@uneatlantico.es creators_id: creators_id: maurizio.battino@uneatlantico.es creators_id: francesca.giampieri@uneatlantico.es title: Can the phenolic compounds of Manuka honey chemosensitize colon cancer stem cells? A deep insight into the effect on chemoresistance and self-renewal ispublished: pub subjects: uneat_sn divisions: uneatlantico_produccion_cientifica divisions: unincol_produccion_cientifica divisions: uninimx_produccion_cientifica divisions: uninipr_produccion_cientifica full_text_status: none abstract: Manuka honey, which is rich in pinocembrin, quercetin, naringenin, salicylic, p-coumaric, ferulic, syringic and 3,4-dihydroxybenzoic acids, has been shown to have pleiotropic effects against colon cancer cells. In this study, potential chemosensitizing effects of Manuka honey against 5-Fluorouracil were investigated in colonspheres enriched with cancer stem cells (CSCs), which are responsible for chemoresistance. Results showed that 5-Fluorouracil increased when it was combined with Manuka honey by downregulating the gene expression of both ATP-binding cassette sub-family G member 2, an efflux pump and thymidylate synthase, the main target of 5-Fluorouracil which regulates the ex novo DNA synthesis. Manuka honey was associated with decreased self-renewal ability by CSCs, regulating expression of several genes in Wnt/β-catenin, Hedgehog and Notch pathways. This preliminary study opens new areas of research into the effects of natural compounds in combination with pharmaceuticals and, potentially, increase efficacy or reduce adverse effects. date: 2023-11 publication: Food Chemistry volume: 427 pagerange: 136684 id_number: doi:10.1016/j.foodchem.2023.136684 refereed: TRUE issn: 03088146 official_url: http://doi.org/10.1016/j.foodchem.2023.136684 access: close language: en citation: Artículo Materias > Alimentación Universidad Europea del Atlántico > Investigación > Producción Científica Fundación Universitaria Internacional de Colombia > Investigación > Producción Científica Universidad Internacional Iberoamericana México > Investigación > Producción Científica Universidad Internacional Iberoamericana Puerto Rico > Investigación > Producción Científica Cerrado Inglés Manuka honey, which is rich in pinocembrin, quercetin, naringenin, salicylic, p-coumaric, ferulic, syringic and 3,4-dihydroxybenzoic acids, has been shown to have pleiotropic effects against colon cancer cells. In this study, potential chemosensitizing effects of Manuka honey against 5-Fluorouracil were investigated in colonspheres enriched with cancer stem cells (CSCs), which are responsible for chemoresistance. Results showed that 5-Fluorouracil increased when it was combined with Manuka honey by downregulating the gene expression of both ATP-binding cassette sub-family G member 2, an efflux pump and thymidylate synthase, the main target of 5-Fluorouracil which regulates the ex novo DNA synthesis. Manuka honey was associated with decreased self-renewal ability by CSCs, regulating expression of several genes in Wnt/β-catenin, Hedgehog and Notch pathways. This preliminary study opens new areas of research into the effects of natural compounds in combination with pharmaceuticals and, potentially, increase efficacy or reduce adverse effects. metadata Cianciosi, Danila; Armas Diaz, Yasmany; Alvarez-Suarez, José M.; Chen, Xiumin; Zhang, Di; Martínez López, Nohora Milena; Briones Urbano, Mercedes; Quiles, José L.; Amici, Adolfo; Battino, Maurizio y Giampieri, Francesca mail SIN ESPECIFICAR, SIN ESPECIFICAR, SIN ESPECIFICAR, SIN ESPECIFICAR, SIN ESPECIFICAR, nohora.martinez@uneatlantico.es, mercedes.briones@uneatlantico.es, jose.quiles@uneatlantico.es, SIN ESPECIFICAR, maurizio.battino@uneatlantico.es, francesca.giampieri@uneatlantico.es (2023) Can the phenolic compounds of Manuka honey chemosensitize colon cancer stem cells? A deep insight into the effect on chemoresistance and self-renewal. Food Chemistry, 427. p. 136684. ISSN 03088146